Use the Tab and Up, Down arrow keys to select menu items.
Engineering biochemistry covering enzyme kinetics, metabolism and applied molecular biology.
TopicsSection 1: Engineernig Biochemistry (Simone Dimartino)• Introduction to course and my lectures. The cell – organelles• Cell components, biomolecules – Carbs, Organic acids, lipids, fats, steroids, amino acids, proteins• Enzymes 1 – What is an enzyme? Enzyme kinetics – M-M• Enzymes 2 – Enzyme kinectics – Inhibition, examples/design of inhibitors (brief)• Metabolism 1 – Principles of metabolism• Metabolism 2 – How do we know? How do you study metabolic pathways• Metabolism 3 – Examples of metabolic pathways – Glycolysis• Metabolism 4 – Examples of metabolic pathways – The Krebs Cycle• Metabolism 5 – In lecture tutorial – Metabolism problem solving (what was previously taught in the tutorials)• Enzymes 3 – Immobilisation• Enzymes 4 – Industrial Uses• Protein bioinformatics – “How to” web guide, Pymol, Protein calculator, PDB, BLAST Section 2: Genetic Engineering (Dr. Celine Valery)Part 1 – Genetic engineering basicsMolecular biology refresher• The central dogma (from DNA to protein synthesis)• DNA structure and function • Protein structure and functionNA replication and repair• Replication process• Repair and recombination• Natural mutationManipulating and analysing DNA 1: main steps in DNA cloning• DNA extraction techniques• Building recombinant DNA: restriction enzymes and ligation• Cloning vectors (types and choice of)• Transformation into host cellsManipulating and analysing DNA 2: PCR and sequencing• Principles of PCR and site-directed mutagenesis• DNA sequencing • Illustration: PCR and sequencing in DNA forensicsProteins: post-translational modifications and folding• Overview of the translation process• Typical post-translational modifications (and reasons for), illustration: formation of disulfide bridges during the production of insulin• The importance of folding: example of pathologies related to protein misfolding (focus on neurodegenerative diseases)• Analysing proteins, classical techniques (including folding analysis)Part 2 - Medical and industrial applicationsOverview of protein expression systems• Currently marketed recombinant products and corresponding expression systems (recombinant therapeutic proteins, antibodies, vaccines,..)• Overview and comparison of expression systems (bacteria, yeasts vs CHO & mammalian), tools for host selection (process requirement and cost criteria)Study case 1 : producing recombinant therapeutics• Production of insulin from bacterial cells • Examples of protein therapeutics production from mammalian cellsStudy case 2 : genetic engineering and viruses• Production of recombinant hepatitis B vaccine from yeast cells• Viral vectors and gene therapyGenetic engineering and biomedical research• Model of diseases and gene knockout engineering• Protein tracking strategies: fusion genes, example of green-fluorescent protein• Protein expression studies: manipulating gene promoters Transgenic plants and genetically modified foods• Transgenic plant proteins: examples and process of production• Transgenic plants: complex systems• Genetically modified foods: gene-implantation technologies (soil bug Agrobacterium tumefaciens, electroporation, gene guns)• Currently marketed modified crops, and global presenceAnimal transgenesis• Gene transfer strategies in animal transgenesis• Transgenic animals and their current use (medicine and industrial)• Study case 1: “pharming” - transgenic animal proteins (example of anti-cancer antibodies from milk products)• Study 2: transgenesis in the improvement of production traits (examples of wool and milk)Review Section 3: Industrial Microbiology (Dr. Gabriel Visnovsky and Dr Jason Ryan)• Prokaryotes & Eukaryotes• Microbial metabolism • Culture media• Screening, Isolation, Preservation and Improvement of Microorganisms• Inoculum preparation and Sterilization• Culture medium design for industrial fermentations• Culture medium design for industrial fermentations• Microbial growth kinetics• Microbial stoichiometry• Bioreactors design and scale up• Case study 1• Case study 2
ENCH281 or subject to approval of the Director of Studies
ENCH323
Gabriel Visnovsky
Block 1: Dr. Simone Dimartino (simone.dimartino@.canterbury.ac.nz) Block 2: Dr. Celine Valery (celine.valery@canterbury.ac.nz) Block 3: Dr. Gabriel Visnovsky (gabriel.visnovsky@canterbury.ac.nz)
Ratledge, Colin. , Kristiansen, B; Basic biotechnology ; 3rd ed; Cambridge University Press, 2006.
Shuler, Michael L. , Kargi, Fikret; Bioprocess engineering : basic concepts ; 2nd ed; Prentice Hall, 2002.
ConcernsStudents with concerns about the course should contact any of the teachers listed above, the 3rd Pro Director of Studies (Dr. Chris Williamson), or the Head of Department.General Policies of the DepartmentStudents may obtain the general policies of the University on matters such as the aegrotat applications, appeals procedures, reconsideration of grades and special provision for students with disabilities from the University Calendar. The Department assessment details, Departmental Safety Handbook, Electrical Safety Supplement and Disposal of Chemical Wastes Policy are distributed to the students at the beginning of the new year.
Domestic fee $808.00
International fee $4,550.00
* All fees are inclusive of NZ GST or any equivalent overseas tax, and do not include any programme level discount or additional course-related expenses.
For further information see Chemical and Process Engineering .