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This course focuses on the priciples of bioseparation processes: cellular fractionation, filtration, chromatography, electrophoresis, immunoaffinity techniques and their application to various types of biomolecules. It includes a hands-on laboratory to separate a target microbially-produced protein by chromatography and assay the purity by electrophoresis.
• Biomolecular properties (6 lectures)• Cellular fractionation and cell disruption techniques (5 lectures)• Liquid chromatography separations (7 lectures)• Electrophoresis (6 lectures)• Immunoaffinity methods (4 lectures)• Mammalian cell separations (2 lectures)• Bioprocess case studies (5 lectures)
At the end of this course, students will:Have an overview of the separation and purification of biological materials.Understand how to exploit biological molecular properties for their separation.Obtain an overview of bioseparation principles to develop bioseparation strategies for complex biological mixtures.Understand and apply the principles of process scale-up in bioseparation processes.Apply optimization and scale-up principles to design or improve a bioseparation process.
ENGR407
Students must attend one activity from each section.
Rachael Wood
Carlo Carere
36 lectures plus 2 labs. Students are expected to spend approximately 2 hours per week reviewing lecture content and preparing for labs, and an additional 10 hours per assignment.
There are no assigned textbooks for this course. Textbooks you may find interesting/useful include:Bioprocess Engineering Principles (Pauline Doran)Chemical Engineering Design (Sinnott Towler)Bioprocess Engineering: Basic Concepts (Michael Schuler)
All information about academic policies (e.g. special consideration, dishonest practice) can be found on the ENCH-Undergraduate LEARN page .
This course is required to complete the Bioprocess Engineering minor in Chemical and Process Engineering. Prerequisites: ENGR407
Domestic fee $1,197.00
International fee $6,000.00
* All fees are inclusive of NZ GST or any equivalent overseas tax, and do not include any programme level discount or additional course-related expenses.
For further information see Chemical and Process Engineering .